Rhea Sarah DSouza

Developed strong research skills in academic environment and expertise in molecular biology, biochemistry, and public skill management. Looking to transition into the scientific field, leveraging critical thinking and problem-solving abilities. Known for collaborative teamwork, adaptability, and delivering impactful results. Seeking opportunities to expand on these skills in a dynamic and challenging setting.

Genomic/Proteomic and Biochemical Assays: DNA/RNA extraction, Gel Electrophoresis, RT-PCR, qPCR, Recombinant DNA, Cloning, DNA sequencing, Protein Purification, PAGE, Western Blotting, Bacterial Culture

Cell culture: Proficient in human cell line handling and culture, including Hela cells, HEK293T cells, HUVEC cells.,Transient Transfection, Lentiviral transfection, Crispr-Cas9 for generating clones, Engineered vector sequences, establishing the experimental foundation and execution of protein purification for downstream analyses, 

Purification skills: AKTA Chromatography, FACS Analysis (using FlowJo), UV/Visible Spectroscopy

Animal model: Using zebrafish for toxicological and developmental assays, Maintenance of zebrafish, Hemoglobin staining, Probe preparation, Whole mount in situ hybridization (WISH)

Bioinformatics:  BioEdit, UniProt, ClustalW, AlphaFold, PDB-Protein Data Bank, Snapgene

• Poster presentation at MBSJ 2022, Molecular Biology Society, Japan, 12/01/22 - 12/03/22, Chiba, Japan
• Poster presentation at BSS, International student seminar by Graduate School of Biostudies, Kyoto University, 03/01/23
• Volunteered and helped organize the Cell Death Colloqium at iCeMS, Kyoto University, 20/01/25-21/01/25

• MEXT Honors scholarship, JASSO, 04/24 - 03/25
• Support for Pioneering graduate Students, Kyoto University Graduate Division, 04/22 - 10/22
• MEXT by SGU scholarship, JASSO, 04/21 - 03/22
• MEXT Honors scholarship, JASSO, 04/20 - 03/21

Playing the guitar, Travelling and Photography