Summary
Overview
Work History
Education
Skills
Publication
Timeline
ResearchAssistant

John Benson Dy

Osaka City

Summary

Enthusiastic PhD in biotechnology graduate with 5-year experience in recombinant protein production in mammalian cell lines. Extremely adaptable to different challenges and problems faced during the research process. Ability to formulate new methods to reach the goal of the research. Proficient in molecular biology techniques such as molecular cloning, protein production, analysis, and purification. Experienced in analytical instruments such as HPLC, LC-MS/MS, and MALDI-ToF MS. Capable of utilizing CRISPR/Cas9 as a gene editing tool.

Overview

5
5
years of professional experience
13
13
years of post-secondary education
4
4
Languages

Work History

Research Assistant

Osaka University
Osaka
2022.10 - 2023.02

Assistant Professor

National University
Manila
2014.11 - 2016.10
  • Research coordinator
  • Program chair of the College of Pharmacy

Research Assistant

Institute of Pharmaceutical Sciences, National Institute of Health
Manila
2014.05 - 2014.09

Pharmacist

ACPAK, Inc
Manila
2011.07 - 2014.01
  • Passed various audits such as GMP and licensing inspection from Unilever Philippines and FDA Philippines

Education

Ph.D. - Biotechnology

Osaka University
Osaka, Japan
2019.05 - 2023.05

Master of Science - Biotechnology

Osaka University
Osaka, Japan
2017.05 - 2019.05

Master of Science - Pharmacy

University of Santo Tomas
Manila, Philippines
2011.05 - 2014.05

Bachelor of Science - Pharmacy

University of Santo Tomas
Manila, Philippines
2006.05 - 2010.05

Skills

Microsoft office tools

Molecular cloning

Mammalian cell culture

Recombinant protein production

Protein Purification

SDS-PAGE/Western blot

ELISA

Gene editing (CRISPR/Cas9

SEC-HPLC

Glycan Analysis (N- and O-glycan)

RT-qPCR

LC-MS/MS and HPLC

Publication

     

John   Benson D. Choa, Tadahiro Sasaki, Hiroyuki Kajiura, Kazuyoshi   Ikuta, Kazuhito Fujiyama, Ryo Misaki: Effects of various disaccharide   adaptations on recombinant IgA1 production in CHO-K1 suspension cells.   Cytotechnology (9th/Feb/2023, Accepted)

John   Benson D. Choa, Hiroyuki Kajiura, Kazuhito Fujiyama, Ryo   Misaki: N-glycan and O-glycan analysis of recombinant IgA1 produced on various disaccharide-adapted CHO-K1 suspension cells. (In preparation) 

John   Benson D. Choa, Roanne V. Lu, Mark Anteo D. Nombrado (2016). Anti-obesity Activity of Broussonetia luzonicus (Moraceae). Journal of Chemical and Pharmaceutical Research. 8(2): 783-788

Choa John Benson D., Lu Roanne V., Nombrado Mark A., Rayos Garina   Kaye R., Invento Chelsea Dae and Castañeda Gerald (2016). Phytochemical   screening of Broussonetia luzonicus (Moraceae) leaves. Journal of Chemical and Pharmaceutical Research. 8(2): 335-338

John Benson D. Choa, Aleth L. Dacanay, and Mario A. Tan (2014). Antibacterial and mosquito larvicidal activity of Ficus casiguranensis and Ficus   camarinensis leaves. Journal of Chemical and Pharmaceutical Research. 6(5): 1351-1354

Timeline

Research Assistant

Osaka University
2022.10 - 2023.02

Ph.D. - Biotechnology

Osaka University
2019.05 - 2023.05

Master of Science - Biotechnology

Osaka University
2017.05 - 2019.05

Assistant Professor

National University
2014.11 - 2016.10

Research Assistant

Institute of Pharmaceutical Sciences, National Institute of Health
2014.05 - 2014.09

Pharmacist

ACPAK, Inc
2011.07 - 2014.01

Master of Science - Pharmacy

University of Santo Tomas
2011.05 - 2014.05

Bachelor of Science - Pharmacy

University of Santo Tomas
2006.05 - 2010.05
John Benson Dy